CCNB1IP1 Knockout cell line (SH-SY5Y)

CCNB1IP1 Knockout cell line (SH-SY5Y) Order

Catalog#

YKO-H1154

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

CCNB1IP1 Knockout cell line (SH-SY5Y)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2-1:3

Species

Human

Gene Name

CCNB1IP1

Gene ID

57820

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:SH-SY5Y			STR Info (Cell bank) Cell Line:SH-SY5Y
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	11	11		11	11
D7S820	7	10		7	10
D16S539	8	13		8	13
VWA	14	18		14	18
TH01	7	10		7	10
AMEL	X	X		X	X
TPOX	8	11		8	11
CSF1PO	11	11		11	11
D12S391	18	22
FGA	23.2	24
D2S1338	17	19
D21S11	31	31.2
D18S51	13	16
D8S1179	15	15
D3S1358	15	16
D6S1043	12	18
PENTAE	7	11
D19S433	13	14
PENTAD	10	12
D1S1656	12	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CCNB1IP1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SH-SY5Y cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CCNB1IP1 gene knockout CCNB1IP1 Knockout cell line (SH-SY5Y) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

CCNB1IP1 Knockout cell line (SH-SY5Y)

Catalog#

YKO-H1154

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Human Neuroblastoma Cell Line(SH-SY5Y)
inquiry More details

Catalog#: YC-D014

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

CCNB1IP1 Knockout cell line (Hep G2)
inquiry More details

Organism:

Gene Name: CCNB1IP1

Gene ID: 57820

Size: 1*10^6

Catalog#: YKO-HT4471

YKO-HT4471()
CCNB1IP1 Knockout cell line (HeLa)
inquiry More details

Organism: Human

Gene Name: CCNB1IP1

Gene ID: 57820

Size: 1*10^6

Catalog#: YKO-XN86625

YKO-XN86625(HeLa)
CCNB1IP1 Knockout cell line (HCT 116)
inquiry More details

Organism: Human

Gene Name: CCNB1IP1

Gene ID: 57820

Size: 1*10^6

Catalog#: YKO-XN86624

YKO-XN86624(HCT 116)

Related Popular Products

Wild-type Cell Lines

Human Neuroblastoma Cell Line(SH-SY5Y)

Price (USD): -

Catalog#: YC-D014

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

CCNB1IP1 Knockout cell line (Hep G2)

Organism:

Gene Name: CCNB1IP1

Gene ID: 57820

Size: 1*10^6

Catalog#: YKO-HT4471

inquiry More details
CCNB1IP1 Knockout cell line (HeLa)

Organism: Human

Gene Name: CCNB1IP1

Gene ID: 57820

Size: 1*10^6

Catalog#: YKO-XN86625

inquiry More details
CCNB1IP1 Knockout cell line (HCT 116)

Organism: Human

Gene Name: CCNB1IP1

Gene ID: 57820

Size: 1*10^6

Catalog#: YKO-XN86624

inquiry More details

CCNB1IP1 Knockout cell line (SH-SY5Y)

Ubigene